Journal: Cells

Article Title: Regional Variation of Gap Junctional Connections in the Mammalian Inner Retina

doi: 10.3390/cells10092396

Figure Lengend Snippet: List of key resources.

Article Snippet: mouse monoclonal anti-calbindin , Synaptic Systems, Göttingen, German , 214011.

Techniques: Plasmid Preparation, Laser-Scanning Microscopy, Software

Journal: Journal of neuroinflammation

Article Title: Neuroinflammation causes mitral cell dysfunction and olfactory impairment in a multiple sclerosis model.

doi: 10.1186/s12974-025-03388-5

Figure Lengend Snippet: Fig. 3 Structural analyses show cell-type specific neuronal vulnerability during EAE. A Representation of the most prominent layers in the main olfactory bulb. Glomerular layer = GL, external plexiform layer = EPL, mitral cell layer = MCL, internal plexiform layer = IPL, granular cell layer = GCL. B–G Representative immunofluorescence images and quantitative measurement of different cell populations according to their main cell markers measured in the olfactory bulb per mm2 in healthy and EAE mice (n = 5–8 per group): B Tyrosine hydroxylase (TH), C Calbindin, D Calretinin, E Parvalbumin (PV), F NeuN and G Doublecortin (DCX). The analyzed layers are described and colored according to the representative image in A. Scale bars = 100 µm. Bars show mean values ± s.e.m. Statistical analysis was performed by unpaired, two-tailed Student’s t-test; *P < 0.05

Article Snippet: Cryosections of olfactory bulb were incubated in 10% of normal donkey serum containing 0.1% of Triton X-100 and were subsequently stained with antibodies against the following antigens: Calbindin (chicken, Synaptic Systems; Cat# 214,006, RRID: AB_2619903), Calretinin (chicken, Synaptic Systems; Cat# 214,106, RRID: AB_2619909), Doublecortin (rabbit, Abcam,; Cat# 18,723, RRID: AB_732011), HuC/D (mouse, Molecular Probes; Cat# A-21271, RRID: AB_221448), NeuN (chicken, Millipore; Cat# ABN91, RRID: AB_11205760), Reelin (mouse, Millipore; Cat# MAB5364, RRID: AB_2179313), PSD95 (mouse, Abcam; Cat# ab2723, RRID: AB_303248), SMI31 (mouse, BioLegend; Cat# 801,601, RRID: AB_2715851), SMI32 (mouse, BioLegend; Cat# 801,701, RRID: AB_2715852), Synapsin 1 (guinea pig, Synaptic Systems; Cat# 106 011, RRID:AB_2619772), and Tyrosine Hydroxylase (mouse, Synaptic Systems; Cat# 213104, RRID:AB_2619897), As secondary antibodies, we used Alexa Fluor 488–coupled donkey antibodies recognizing chicken IgG (1:600, Jackson Immunoresearch; Cat# 703–545-155, RRID: AB_2340375), rabbit IgG (1:600, Jackson Immunoresearch; Cat# 711–546-152, RRID: AB_2340619) and mouse IgG (1:600, Abcam; Cat# ab150105, RRID: AB_2732856), Alexa Fluor 647–coupled donkey antibodies recognizing rat IgG(1:600, Abcam; Abcam; Cat# ab150159, RRID: AB_2566823) and rabbit IgG (1:600, Abcam; Cat# ab150075, RRID: AB_2752244).

Techniques: Immunofluorescence, Two Tailed Test

Journal: Journal of neuroinflammation

Article Title: Neuroinflammation causes mitral cell dysfunction and olfactory impairment in a multiple sclerosis model.

doi: 10.1186/s12974-025-03388-5

Figure Lengend Snippet: Fig. 5 SnRNA-Seq of the olfactory bulb reveals a heterogeneity of neuronal cell types. A Study design of single-nucleus RNA sequencing approach of neuronal nuclei in the olfactory bulb of healthy and acute EAE mice. Visualization done with Biorender. B UMAP plot with a total of 73,835 cells from six mice revealing 15 different clusters of mostly neuronal cells in the olfactory bulb. PGC = periglomerular cells, M/T = mitral/tufted cells, GC = granular cells, EPL-IN = interneurons of the external plexiform layer, MyOligos = myelinating oligodendrocytes, OEC = olfactory ensheathing cells. C Feature plots of prominent cell types including mature neurons, immature neurons, inhibitory neurons, excitatory neurons and two groups of prominent cell populations as defined by the depicted marker gene: Periglomerular cells with calretinin(Calb2)-positive, TH-positive and calbindin(Calb1)-positive cells as well as mitral/tufted cell clusters, which are further analyzed in Fig. 6. D Dot plot of the marker genes of the 15 distinct clusters

Article Snippet: Cryosections of olfactory bulb were incubated in 10% of normal donkey serum containing 0.1% of Triton X-100 and were subsequently stained with antibodies against the following antigens: Calbindin (chicken, Synaptic Systems; Cat# 214,006, RRID: AB_2619903), Calretinin (chicken, Synaptic Systems; Cat# 214,106, RRID: AB_2619909), Doublecortin (rabbit, Abcam,; Cat# 18,723, RRID: AB_732011), HuC/D (mouse, Molecular Probes; Cat# A-21271, RRID: AB_221448), NeuN (chicken, Millipore; Cat# ABN91, RRID: AB_11205760), Reelin (mouse, Millipore; Cat# MAB5364, RRID: AB_2179313), PSD95 (mouse, Abcam; Cat# ab2723, RRID: AB_303248), SMI31 (mouse, BioLegend; Cat# 801,601, RRID: AB_2715851), SMI32 (mouse, BioLegend; Cat# 801,701, RRID: AB_2715852), Synapsin 1 (guinea pig, Synaptic Systems; Cat# 106 011, RRID:AB_2619772), and Tyrosine Hydroxylase (mouse, Synaptic Systems; Cat# 213104, RRID:AB_2619897), As secondary antibodies, we used Alexa Fluor 488–coupled donkey antibodies recognizing chicken IgG (1:600, Jackson Immunoresearch; Cat# 703–545-155, RRID: AB_2340375), rabbit IgG (1:600, Jackson Immunoresearch; Cat# 711–546-152, RRID: AB_2340619) and mouse IgG (1:600, Abcam; Cat# ab150105, RRID: AB_2732856), Alexa Fluor 647–coupled donkey antibodies recognizing rat IgG(1:600, Abcam; Abcam; Cat# ab150159, RRID: AB_2566823) and rabbit IgG (1:600, Abcam; Cat# ab150075, RRID: AB_2752244).

Techniques: RNA Sequencing, Marker